Eurasian Journal of Soil Science
Volume 15, Issue 4, Oct 2026, Pages 538-552
DOI: 10.18393/ejss.2005110
Stable URL: https://ejss.fesss.org/manuscript.php?id=4576
Copyright © 2026 The authors and Federation of Eurasian Soil Science Societies
Isolation of indigenous soil bacteria with potential application for diuron removal
Abstract
Diuron is a widely used herbicide in sugarcane, cotton, coffee, and citrus production and is classified as a priority pollutant. Physical and chemical remediation of pesticide-contaminated sites is often technically complex and costly. Microorganism-mediated bioremediation offers an environmentally compatible and cost-effective alternative, particularly when pollutant-specific indigenous microorganisms are used. In this study, microorganisms were isolated from diuron-amended soil microcosms, and their diuron removal capacities were evaluated under different pH and temperature conditions. Fourteen bacterial isolates were initially screened, of which six capable of growing in diuron-supplemented medium were selected. Isolates 2 and 6 achieved the highest removal efficiencies, reaching 53% and 51%, respectively, within 3 days. A mixed microbial culture achieved 60% removal, suggesting potential complementary interactions among the isolates. Based on 16S rRNA gene sequencing, isolate 2 showed 97–99% sequence identity to Bacillus sp., whereas isolate 6 showed 97–99% identity to Cellulomonas sp. The study aimed to identify bacterial strains with potential application in future pesticide bioremediation. Indigenous isolates with high pollutant-removal capacity may improve bioremediation performance in their native environments because they are adapted to prevailing local conditions.
Keywords
Bio-removal, Cellulomonas, diuron, indigenous soil microorganisms, pesticides.
References
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Abstract
Diuron is a widely used herbicide in sugarcane, cotton, coffee, and citrus production and is classified as a priority pollutant. Physical and chemical remediation of pesticide-contaminated sites is often technically complex and costly. Microorganism-mediated bioremediation offers an environmentally compatible and cost-effective alternative, particularly when pollutant-specific indigenous microorganisms are used. In this study, microorganisms were isolated from diuron-amended soil microcosms, and their diuron removal capacities were evaluated under different pH and temperature conditions. Fourteen bacterial isolates were initially screened, of which six capable of growing in diuron-supplemented medium were selected. Isolates 2 and 6 achieved the highest removal efficiencies, reaching 53% and 51%, respectively, within 3 days. A mixed microbial culture achieved 60% removal, suggesting potential complementary interactions among the isolates. Based on 16S rRNA gene sequencing, isolate 2 showed 97–99% sequence identity to Bacillus sp., whereas isolate 6 showed 97–99% identity to Cellulomonas sp. The study aimed to identify bacterial strains with potential application in future pesticide bioremediation. Indigenous isolates with high pollutant-removal capacity may improve bioremediation performance in their native environments because they are adapted to prevailing local conditions.
Keywords: Bio-removal, Cellulomonas, diuron, indigenous soil microorganisms, pesticides.
References
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Barathidasan, K., Reetha, D., Milton, D.J., Sriram, N., Govindammal, M., 2014. Biodegradation of chlorpyrifos by co-culture of Cellulomonas fimi and Phanerochaete chrysosporium. African Journal of Microbiology Research 8(9): 961–966.
Belz, R.G., Carbonari, C.A., Duke, S.O., 2022. The potential influence of hormesis on evolution of resistance to herbicides. Current Opinion in Environmental Science & Health 27: 100360.
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Borowik, A., Wyszkowska, J., Zaborowska, M., Kucharski, J., 2023. Microbial diversity and enzyme activity as indicators of permethrin-exposed soil health. Molecules 28(12): 4756.
Catalá-Icardo, M., López-Paz, J.L., Peña-Bádena, A., 2011. FI-photoinduced chemiluminescence method for diuron determination in water samples. Analytical Sciences 27(3): 291–296.
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Coelho-Moreira, J. da S., Brugnari, T., Sá-Nakanishi, A.B., Castoldi, R., de Souza, C.G.M., Bracht, A., Peralta, R.M., 2018. Evaluation of diuron tolerance and biotransformation by the white-rot fungus Ganoderma lucidum. Fungal Biology 122(6): 471–478.
Cristina dos Reis, F., Mielke, K.C., Mendes, K.F., Nogueira de Sousa, R., Heluany, M.H., Tornisielo, V.L., Filho, R.V., 2023. Diuron, hexazinone, and sulfometuron-methyl applied alone and in mixture in soils: Distribution of extractable residue, bound residue, biodegradation, and mineralization. Heliyon 9(7): e17817.
Cullington, J.E., Walker, A., 1999. Rapid biodegradation of diuron and other phenylurea herbicides by a soil bacterium. Soil Biology and Biochemistry 31(5): 677–686.
Dahal, R.H., Kim, J., Kim, D.U., Dong, K., Hong, Y., Chaudhary, D.K., 2022. Cellulomonas fulva sp. nov., isolated from oil-contaminated soil. International Journal of Systematic and Evolutionary Microbiology 72: 005209.
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Domingues, S.Z., Timmers, L.F.S.M., Granada, C.E., 2022. Cellulase production by bacteria is a strain-specific characteristic with high biotechnological potential: A review of the cellulosome of highly studied strains. Cellulose 29(15): 8065–8083.
Duc, H.D., Thuy, N.T.D., Thanh, L.U., Tuong, T.D., Oanh, N.T., 2022. Degradation of diuron by a bacterial mixture and shifts in the bacterial community during bioremediation of contaminated soil. Current Microbiology 79(1): 11.
Egea, T.C., da Silva, R., Boscolo, M., Rigonato, J., Monteiro, D.A., Grünig, D., da Silva, H., van der Wielen, F., Helmus, R., Parsons, J.R., Gomes, E., 2017. Diuron degradation by bacteria from soil of sugarcane crops. Heliyon 3(12): e00471.
Fucic, A., Duca, R.C., Galea, K.S., Maric, T., Garcia, K., Bloom, M.S., Andersen, H.R., Vena, J.E., 2021. Reproductive health risks associated with occupational and environmental exposure to pesticides. International Journal of Environmental Research and Public Health 18(12): 6576.
Giacomazzi, S., Cochet, N., 2004. Environmental impact of diuron transformation: A review. Chemosphere 56(11): 1021–1032.
Góngora-Echeverría, V.R., García-Escalante, R., Rojas-Herrera, R., Giácoman-Vallejos, G., Ponce-Caballero, C., 2020. Pesticide bioremediation in liquid media using a microbial consortium and bacteria-pure strains isolated from a biomixture used in agricultural areas. Ecotoxicology and Environmental Safety 200: 110734.
Guimarães, A.C.D., Mendes, K.F., dos Reis, F.C., Campion, T.F., Christoffoleti, P.J., Tornisielo, V.L., 2018. Role of soil physicochemical properties in quantifying the fate of diuron, hexazinone, and metribuzin. Environmental Science and Pollution Research 25(13): 12419–12433.
Hao, B., Wu, H., Zhang, S., He, B., 2022. Individual and combined toxicity of microplastics and diuron differs between freshwater and marine diatoms. Science of the Total Environment 853: 158334.
Henn, C., Arakaki, R.M., Monteiro, D.A., Boscolo, M., da Silva, R., Gomes, E., 2020. Degradation of the organochlorinated herbicide diuron by rainforest basidiomycetes. BioMed Research International Article ID 5324391.
Holmes, G., 2014. Australia’s pesticide environmental risk assessment failure: The case of diuron and sugarcane. Marine Pollution Bulletin 88(1–2): 7–13.
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How to cite
Engin, B., Yılmaz, H., Erdoğan, E., Özkan, M., 2026. Isolation of indigenous soil bacteria with potential application for diuron removal. Eurasian J. Soil Sci. 15(4): 538-552. DOI : 10.18393/ejss.2005110
Citation Styles
CLASSICAL
Engin, B., Yılmaz, H., Erdoğan, E., Özkan, M., 2026. Isolation of indigenous soil bacteria with potential application for diuron removal. Eurasian J. Soil Sci. 15(4): 538-552. DOI : 10.18393/ejss.2005110
APA
Engin, B., Yılmaz, H., Erdoğan, E., & Özkan, M. (2026). Isolation of indigenous soil bacteria with potential application for diuron removal Eurasian Journal of Soil Science, 15(4): 538-552. DOI : 10.18393/ejss.2005110
MLA
Engin, B., Yılmaz, H., Erdoğan, E., and Özkan, M. "Isolation of indigenous soil bacteria with potential application for diuron removal" Eurasian Journal of Soil Science, 15.4 (2026) DOI : 10.18393/ejss.2005110
TURABIAN
Engin, B., Yılmaz, H., Erdoğan, E., and Özkan, M. "Isolation of indigenous soil bacteria with potential application for diuron removal" Eurasian Journal of Soil Science, 15(Oct 2026):538-552 DOI : 10.18393/ejss.2005110
IEEE
B. Engin., H. Yılmaz., E. Erdoğan., M. Özkan. "Isolation of indigenous soil bacteria with potential application for diuron removal" Eurasian J. Soil Sci., vol. 15, no. 4, pp. 538-552 (Oct 2026) DOI : 10.18393/ejss.2005110
ISO690
ENGIN, Berk Emre ; YILMAZ, Hilal ; ERDOĞAN, Esra Meşe ; ÖZKAN, Melek Isolation of indigenous soil bacteria with potential application for diuron removal. Eurasian Journal of Soil Science, (2026), 15.4:538-552.DOI : 10.18393/ejss.2005110
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Publication Information
- Article first published online: 28 Temmuz 2026
- Manuscript Accepted: 16 Temmuz 2026
- Manuscript Received: 16 Şubat 2026
- DOI: 10.18393/ejss.2005110
- Stable URL: https://ejss.fesss.org/manuscript.php?id=4576
