Eurasian Journal of Soil Science

Volume 11, Issue 1, Jan 2022, Pages 10-16
DOI: 10.18393/ejss.974224
Stable URL: http://ejss.fess.org/10.18393/ejss.974224
Copyright © 2022 The authors and Federation of Eurasian Soil Science Societies



Identification of humic substances on the transformation of an organic substrate

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Bezuglova,O., Komarov,A., Komarov ,A., 2022. Identification of humic substances on the transformation of an organic substrate. Eurasian J Soil Sci 11(1):10-16. DOI : 10.18393/ejss.974224
Bezuglova,O.,Komarov,A.,& Komarov ,A. Identification of humic substances on the transformation of an organic substrate Eurasian Journal of Soil Science, 11(1):10-16. DOI : 10.18393/ejss.974224
Bezuglova,O.,Komarov,A., and ,Komarov ,A."Identification of humic substances on the transformation of an organic substrate" Eurasian Journal of Soil Science, 11.1 (2022):10-16. DOI : 10.18393/ejss.974224
Bezuglova,O.,Komarov,A., and ,Komarov ,A. "Identification of humic substances on the transformation of an organic substrate" Eurasian Journal of Soil Science,11(Jan 2022):10-16 DOI : 10.18393/ejss.974224
O,Bezuglova.A,Komarov.A,Komarov "Identification of humic substances on the transformation of an organic substrate" Eurasian J. Soil Sci, vol.11, no.1, pp.10-16 (Jan 2022), DOI : 10.18393/ejss.974224
Bezuglova,Olga ;Komarov,Andrey ;Komarov ,Alexey Identification of humic substances on the transformation of an organic substrate. Eurasian Journal of Soil Science, (2022),11.1:10-16. DOI : 10.18393/ejss.974224

How to cite

Bezuglova, O., Komarov, A., Komarov , A., 2022. Identification of humic substances on the transformation of an organic substrate. Eurasian J. Soil Sci. 11(1): 10-16. DOI : 10.18393/ejss.974224

Author information

Olga Bezuglova , Southern Federal University, Academy of Biology and Biotechnology, 344090, Rostov-on-Don, Russia
Andrey Komarov , Agrophysical Research Institute, pr. Grazhdanskiy 14, 195220, St. Petersburg, Russia
Alexey Komarov , Leningrad Research Institute of Agriculture "Belogorka", Institutskaya street, Leningrad region, Gatchinsky district, Belogorka village, 188338 Russia

Publication information

Article first published online : 25 Jul 2021
Manuscript Accepted : 20 Jul 2021
Manuscript Received: 08 Feb 2021
DOI: 10.18393/ejss.974224
Stable URL: http://ejss.fesss.org/10.18393/ejss.974224

Abstract

The organic matter of the soil and humus are heterogeneous in composition. That is why the nature of soil organic matter has not yet been fully understood and causes many discussions. The purpose of this work is to study the identification features of humus in the process of peat's transformation. The results of a five-year experiment studying the processes of organic matter transformation in organic-mineral substrate based on peat growing coniferous seedlings in greenhouses are presented. In the dynamics of the group and fractional composition of humus for several years the biochemical essence of the humification process is revealed. To extract specific humic substances from the organogenic substrate, we used the method of fractioning humus into groups and fractions, which is a stepwise sequential extraction using solutions of sodium hydroxide and sulfuric acid. It was found that the organic matter of the substrate passes through three stages of transformation: hydrolysis of organic products → initial transformation → humification of lignin structures. There is a significant change in the ratio of the three main groups of humus in favor of humic acids at the third stage. In general, the system is tends to its most stable state: aromatic structures are copolymerized, the core of humic substances is densified, and humus gradually "matures".

Keywords

Fulvic acid, humic acid, humus formation, peat.

Corresponding author

References

Abakumov, E., Fujitake, N., Takashi, K., 2009. Humus and humic acids of luvisol and cambisol of jiguli ridges, Samara Region, Russia. Applied and Environmental Soil Science Article ID 671359. 

Alexandrova, L.N., 1980. Soil organic matter and the processes of its transformation. Nauka Publishing House, Leningrad, Russia. 290p.

Aliev, S.A., 1978. Ecology and energy of biochemical transformation of soil organic matter. ELM Publishing House, Azerbaijan. 252p. [in Russian].

Bezuglova, O., 2019. Molecular structure of humus acids in soils. Journal of Plant Nutrition and Soil Science 182: 676-682.   

Bezuglova, O.S., Gorovtsov, A.V., Polienko, E.A. Zinchenko, V.E., Grinko, A.V., Lykhman, V.A., Dubinina, M.N., Demidov, A., 2019. Effect of humic preparation on winter wheat productivity and rhizosphere microbial community under herbicide-induced stress. Journal of Soils and Sediments 19: 2665–2675.

de Melo, G.B.A., Motta, F.L., Santana, M.H.A., 2016. Humic acids: Structural properties and multiple functionalities for novel technological developments. Materials Science and Engineering C 62: 967-974.

Dospekhov, B.A., 1985. Methods of field experiments. Agropromizdat Publishing House, Moscow, 351p. [in Russian].

Dubovik, E.V., Cherkasov, G.N., 2013. Group and fractional composition of humus of typical chernozem in the geomorphological profile on polar-facing slopes. Russian Agricultural Science 39: 156–158.

Fedotov, G.N., Dobrovolskiy, G.V., 2012. Possible ways of nanostructure development in soil gels. Eurasian Soil Science 45: 811–822.

Gmurman, V.E., 2004. Probability theory and mathematical statistics: Textbook for universities. 10th edition, Stereotyped. Higher School. Moscow, Russia. 479p.

Hristov, B., Filcheva, E., 2017. Soil organic matter content and composition in different pedoclimatic zones of Bulgaria. Eurasian Journal of Soil Science 6(1): 65-74.

Komarov, An. A., Komarov, Al. A., 2017. The hypothesis of the manifestation of the physiological activity of humic substances in the aspect of the humification process. Agrochemical Bulletin 6: 49–54. [in Russian].

Kroyan, S.Z., 2018. The contemporary state of the humus nutrion of the cambisols of Republic of Armenia. Advances in Biotechnology and Microbiology 11(3): 555815.

Lapin, Yu. P., Nollendorf, V.F., 1975. The influence of the level of mineral nutrition on the radish crop in a peat substrate. In: Trace elements in the complex of mineral nutrition of plants. Zinatne Publishing House, Riga, Latvia, pp. 75–83.

Lehmann, J., Kleber, M., 2015. The contentious nature of soil organic matter. Nature 528: 60–68.

Lehmann, J., Solomon, D., Kinyangi, J., Dathe, L., Wirick, S., Jacobsen, C., 2008. Spatial complexity of soil organic matter forms at nanometre scales. Nature Geoscience 1: 238–242.   

Marchik, T.P., Efremov, A.L., 2006. Soil science with the basics of crop production. Grodno, Belarus. 249p. [in Russian].

Matveeva, N.M., Valeeva, A.A., 2012. Statistical processing of the results of field agrochemical studies using the Statgraphics Plus for Windows package: a teaching aid for students of the Faculty of Biology and Soil Science. Kazan University, Kazan. 63p. [in Russian].

Nebbioso, A., Piccolo, A., 2011. Basis of a humeomics science: Chemical fractionation and molecular characterization of humic biosuprastructures. Biomacromolecules 12(4): 1187–1199.   

Nebbioso, A., Piccolo, A., 2012. Advances in humeomics: Enhanced structural identification of humic molecules after size fractionation of a soil humic acid. Analytica Chimica Acta 720: 77–90.  

Orlov, D. S., 1992. Soil Chemistry. Russian Translation, Series 92. Published by CRC Press.

Orlov, D.S, Biryukova, O.N., Rozanova, M.S., 2004. Revised system of the humus status parameters of soils and their genetic horizons. Eurasian Soil Science 37(8): 798–805.

Piccolo, A., 2001. The supramolecular structure of humic substances. Soil Science 166(11): 810–832.

Piccolo, A., 2002. The supramolecular structure of humic substances. A novel understanding of humus chemistry and implications in soil science. Advances in Agronomy 75: 57–134.

Piccolo, A., Spaccini, R., Savy, D., Drosos, M., Cozzolino, V., 2019. The Soil Humeome: Chemical Structure, Functions and Technological Perspectives. In: Sustainable Agrochemistry. Vaz Jr. S. (Ed.). Springer, Cham, pp. 183–222.

Pirog, T.P., Iutynska, G.O., Leonova, N.O., Beregova, K.A. Shevchuk, T.A., 2018. Microbial synthesis of phytohormones. Biotechnologia Acta 11(1): 1–24.

Ponomareva, V.V., Plotnikova, T.A., 1980. Humus and Soil Formation (Methods and Study Results). Nauka Publishing House, Leningrad, Russia. 222p. [in Russian].

Sannino, F., Piccolo, A., 2013. Effective remediation of contaminated soils by eco-compatible chemical, biological and biomimetic practices. In: Sustainable development in chemical engineering: innovative technologies. Basile, A., Piemonte, V., de Falco, M. (Eds.). Wiley, Chichester, UK. pp. 267–296.

Sbih, M., Karam, A., N’Dayegamiye, A., Bensid, Z., Boukaboub, A., 2012. Dynamic of the active fraction of organic matter in some meadow soils. Eurasian Journal of Soil Science 1(1): 22-27.

Shahin, R.R., Khater, H.A., 2020. Quality and quantity of soil organic matter as affected by the period of organic farming in Sekem farm, Egypt. Eurasian Journal of Soil Science 9(3): 275-281.

Tyurin, I.V., 1951. On the analysis technique for a comparative study of the composition of soil humus or humus. Transactions of the Soil Institute named after V.V. Dokuchaev 38: 23–32. [in Russian].

Abstract

The organic matter of the soil and humus are heterogeneous in composition. That is why the nature of soil organic matter has not yet been fully understood and causes many discussions. The purpose of this work is to study the identification features of humus in the process of peat's transformation. The results of a five-year experiment studying the processes of organic matter transformation in organic-mineral substrate based on peat growing coniferous seedlings in greenhouses are presented. In the dynamics of the group and fractional composition of humus for several years the biochemical essence of the humification process is revealed. To extract specific humic substances from the organogenic substrate, we used the method of fractioning humus into groups and fractions, which is a stepwise sequential extraction using solutions of sodium hydroxide and sulfuric acid. It was found that the organic matter of the substrate passes through three stages of transformation: hydrolysis of organic products → initial transformation → humification of lignin structures. There is a significant change in the ratio of the three main groups of humus in favor of humic acids at the third stage. In general, the system is tends to its most stable state: aromatic structures are copolymerized, the core of humic substances is densified, and humus gradually "matures".

Keywords: Fulvic acid, humic acid, humus formation, peat.

References

Abakumov, E., Fujitake, N., Takashi, K., 2009. Humus and humic acids of luvisol and cambisol of jiguli ridges, Samara Region, Russia. Applied and Environmental Soil Science Article ID 671359. 

Alexandrova, L.N., 1980. Soil organic matter and the processes of its transformation. Nauka Publishing House, Leningrad, Russia. 290p.

Aliev, S.A., 1978. Ecology and energy of biochemical transformation of soil organic matter. ELM Publishing House, Azerbaijan. 252p. [in Russian].

Bezuglova, O., 2019. Molecular structure of humus acids in soils. Journal of Plant Nutrition and Soil Science 182: 676-682.   

Bezuglova, O.S., Gorovtsov, A.V., Polienko, E.A. Zinchenko, V.E., Grinko, A.V., Lykhman, V.A., Dubinina, M.N., Demidov, A., 2019. Effect of humic preparation on winter wheat productivity and rhizosphere microbial community under herbicide-induced stress. Journal of Soils and Sediments 19: 2665–2675.

de Melo, G.B.A., Motta, F.L., Santana, M.H.A., 2016. Humic acids: Structural properties and multiple functionalities for novel technological developments. Materials Science and Engineering C 62: 967-974.

Dospekhov, B.A., 1985. Methods of field experiments. Agropromizdat Publishing House, Moscow, 351p. [in Russian].

Dubovik, E.V., Cherkasov, G.N., 2013. Group and fractional composition of humus of typical chernozem in the geomorphological profile on polar-facing slopes. Russian Agricultural Science 39: 156–158.

Fedotov, G.N., Dobrovolskiy, G.V., 2012. Possible ways of nanostructure development in soil gels. Eurasian Soil Science 45: 811–822.

Gmurman, V.E., 2004. Probability theory and mathematical statistics: Textbook for universities. 10th edition, Stereotyped. Higher School. Moscow, Russia. 479p.

Hristov, B., Filcheva, E., 2017. Soil organic matter content and composition in different pedoclimatic zones of Bulgaria. Eurasian Journal of Soil Science 6(1): 65-74.

Komarov, An. A., Komarov, Al. A., 2017. The hypothesis of the manifestation of the physiological activity of humic substances in the aspect of the humification process. Agrochemical Bulletin 6: 49–54. [in Russian].

Kroyan, S.Z., 2018. The contemporary state of the humus nutrion of the cambisols of Republic of Armenia. Advances in Biotechnology and Microbiology 11(3): 555815.

Lapin, Yu. P., Nollendorf, V.F., 1975. The influence of the level of mineral nutrition on the radish crop in a peat substrate. In: Trace elements in the complex of mineral nutrition of plants. Zinatne Publishing House, Riga, Latvia, pp. 75–83.

Lehmann, J., Kleber, M., 2015. The contentious nature of soil organic matter. Nature 528: 60–68.

Lehmann, J., Solomon, D., Kinyangi, J., Dathe, L., Wirick, S., Jacobsen, C., 2008. Spatial complexity of soil organic matter forms at nanometre scales. Nature Geoscience 1: 238–242.   

Marchik, T.P., Efremov, A.L., 2006. Soil science with the basics of crop production. Grodno, Belarus. 249p. [in Russian].

Matveeva, N.M., Valeeva, A.A., 2012. Statistical processing of the results of field agrochemical studies using the Statgraphics Plus for Windows package: a teaching aid for students of the Faculty of Biology and Soil Science. Kazan University, Kazan. 63p. [in Russian].

Nebbioso, A., Piccolo, A., 2011. Basis of a humeomics science: Chemical fractionation and molecular characterization of humic biosuprastructures. Biomacromolecules 12(4): 1187–1199.   

Nebbioso, A., Piccolo, A., 2012. Advances in humeomics: Enhanced structural identification of humic molecules after size fractionation of a soil humic acid. Analytica Chimica Acta 720: 77–90.  

Orlov, D. S., 1992. Soil Chemistry. Russian Translation, Series 92. Published by CRC Press.

Orlov, D.S, Biryukova, O.N., Rozanova, M.S., 2004. Revised system of the humus status parameters of soils and their genetic horizons. Eurasian Soil Science 37(8): 798–805.

Piccolo, A., 2001. The supramolecular structure of humic substances. Soil Science 166(11): 810–832.

Piccolo, A., 2002. The supramolecular structure of humic substances. A novel understanding of humus chemistry and implications in soil science. Advances in Agronomy 75: 57–134.

Piccolo, A., Spaccini, R., Savy, D., Drosos, M., Cozzolino, V., 2019. The Soil Humeome: Chemical Structure, Functions and Technological Perspectives. In: Sustainable Agrochemistry. Vaz Jr. S. (Ed.). Springer, Cham, pp. 183–222.

Pirog, T.P., Iutynska, G.O., Leonova, N.O., Beregova, K.A. Shevchuk, T.A., 2018. Microbial synthesis of phytohormones. Biotechnologia Acta 11(1): 1–24.

Ponomareva, V.V., Plotnikova, T.A., 1980. Humus and Soil Formation (Methods and Study Results). Nauka Publishing House, Leningrad, Russia. 222p. [in Russian].

Sannino, F., Piccolo, A., 2013. Effective remediation of contaminated soils by eco-compatible chemical, biological and biomimetic practices. In: Sustainable development in chemical engineering: innovative technologies. Basile, A., Piemonte, V., de Falco, M. (Eds.). Wiley, Chichester, UK. pp. 267–296.

Sbih, M., Karam, A., N’Dayegamiye, A., Bensid, Z., Boukaboub, A., 2012. Dynamic of the active fraction of organic matter in some meadow soils. Eurasian Journal of Soil Science 1(1): 22-27.

Shahin, R.R., Khater, H.A., 2020. Quality and quantity of soil organic matter as affected by the period of organic farming in Sekem farm, Egypt. Eurasian Journal of Soil Science 9(3): 275-281.

Tyurin, I.V., 1951. On the analysis technique for a comparative study of the composition of soil humus or humus. Transactions of the Soil Institute named after V.V. Dokuchaev 38: 23–32. [in Russian].



Eurasian Journal of Soil Science