Abstract

This study examined microalgal biovolume and taxonomic composition across a restoration chronosequence in sandy post-mining soils and their relationships with abiotic soil properties. The research was conducted in a single sand pit in Central Europe, representing a case study of a typical regional post-mining environment. Samples were collected in winter, spring, and summer from nine experimental plots representing a 4–43-year chronosequence after mining cessation, and from three control plots where mining did not occur. The microalgal biovolume was higher in recently restored plots and lower in older plots. Although biovolume varied seasonally, seasonal changes in taxonomic composition were limited. Biovolume showed a significant negative correlation with organic matter content in all studied seasons, and a similar pattern was observed for Oscillatoriales and Zygnemales, whereas correlations for other taxa varied among seasons and were not consistently significant. In this study, microalgal characteristics did not provide sufficient information for assessing the restoration of sandy post-mining soils under field conditions. However, the decrease in biovolume along the restoration chronosequence, its negative relationship with organic matter content, and the similar relationships observed for taxa identified in this study suggest directions for further investigation. These findings require verification across multiple post-mining sites.

Keywords: Soil microalgae, biovolume, taxonomic composition, restoration chronosequence, seasonal variation, abiotic soil properties.

References

Abinandan, S., Subashchandrabose, S.R., Venkateswarlu, K., Megharaj, M., 2019. Soil microalgae and cyanobacteria: Biotechnological potential in the maintenance of soil fertility and health. Critical Reviews in Biotechnology 39: 981–998.

Andreieva, V.M., 1998. Soil and aerophilic green algae (Chlorophyta: Tetrasporales, Chlorococcales, Chlorosarcinales). Nauka, Moscow. 351p. [in Russian]

Bateman, A., Muñoz-Rojas, M., 2019. To whom the burden of soil degradation and management concerns. In: Advances in Chemical Pollution, Environmental Management and Protection, Volume 4, pp.1–22.

Belnap, J., Büdel, B., Lange, O., 2001. Biological Soil Crusts: Characteristics and Distribution. In: Biological Soil Crusts: Structure, Function, and Management. Belnap, J., Lange, O.L. (Eds.). Vol 150, Springer-Verlag Berlin Heidelberg, pp. 3-30.

Ettl, H., Gärtner, G., 1988. Süßwasserflora von Mitteleuropa 10.: Chlorophyta II. Tetrasporales, Chlorococcales, Gloeodendrales.Fischer Verlag. Stuttgart. 437 p.  [in German]

Frindte, K., Pape, R., Werner, K., Löffler, J., Knief, C., 2019. Temperature and soil moisture control microbial community composition in an arctic–alpine ecosystem along elevational and micro-topographic gradients. The ISME Journal 13(8): 2031–2043.

Ghanei-Bafghi, M.-J., Feiznia, S., Mokhtari, A.R., Jaafari, M., Tavili, A., Khodaeian, Z., 2023. Agricultural soil contamination and degradation near a mining area in an arid region. Journal of Geochemical Exploration 256: 107349.

Hammer, Ø., Harper, D., Ryan, P., 2001. PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4: 1–9.

Hillebrand, H., Dürselen, C.D., Kirschtel, D., Pollingher, U., Zohary, T., 1999. Biovolume calculation for pelagic and benthic microalgae. Journal of Phycology 35: 403–424.

Hu, C.-X., Liu, Y.-D., 2003. Primary succession of algal community structure in desert soil. Acta Botanica Sinica 45: 917–924.

Huang, L.-N., Tang, F.-Z., Song, Y.-S., Wan, C.-Y., Wang, S.-L., Liu, W.-Q., Shu, W.-S., 2011. Biodiversity, abundance, and activity of nitrogen-fixing bacteria during primary succession on a copper mine tailings. FEMS Microbiology Ecology 78: 439–450.

ISO 10390:2021. Soil, treated biowaste and sludge – Determination of pH. Available at [Access date: 20.01.2026]: https://www.iso.org/standard/75243.html

ISO 10523:2008. Water quality — Determination of pH. Available at [Access date: 20.01.2026]: https://www.iso.org/standard/51994.html 

ISO 11465:2025. Sludge and solid environmental matrices — Determination of dry residue or water content and calculation of the dry matter fraction on a mass basis. Available at [Access date: 20.01.2026]: https://www.iso.org/standard/88192.html 

ISO 7888:1985. Water quality — Determination of electrical conductivity. Available at [Access date: 20.01.2026]: https://www.iso.org/standard/14838.html

Jelecevic, A., Sager, M., Vollprecht, D., Puschenreiter, M., Liebhard, P., 2021. Partitioning of heavy metals in different particle-size fractions of soils from former mining and smelting locations in Austria. Eurasian Journal of Soil Science 10(2): 123 - 131.

Johnson, S., Kuske, C., Carney, T., Housman, D., Gallegos-Graves, L., Belnap, J., 2012. Increased temperature and altered summer precipitation have differential effects on biological soil crusts in a dryland ecosystem. Global Change Biology 18: 2583-2593.

Joseph, J., Ray, J.G., 2024. A critical review of soil algae as a crucial soil biological component of high ecological and economic significance. Journal of Phycology 60: 229-253.

Kaštovská, K., Elster, J., Stibal, M., Šantrůčková, H., 2005. Microbial assemblages in soil microbial succession after glacial retreat in Svalbard (High Arctic). Microbial Ecology 50: 396–407.

Komárek, J., 2013. Cyanoprokaryota 3. Heterocytous genera. Süßwasserflora von Mitteleuropa, Vol. 19/3. Springer Spektrum, Berlin, Heidelberg. 1130p.

Komárek, J., Anagnostidis, K., 1998. Cyanoprokaryota 1. Chroococcales. Süßwasserflora von Mitteleuropa, Vol. 19/1. Gustav Fischer Verlag, Stuttgart–Jena. 548p.

Komárek, J., Anagnostidis, K., 2005. Cyanoprokaryota 2. Oscillatoriales. Süßwasserflora von Mitteleuropa, Vol. 19/2. Elsevier Spectrum, Amsterdam. 759p.

Krammer, K., Lange-Bertalot, H., 1986–2004. Bacillariophyceae. Parts 1–4. Gustav Fischer Verlag, Stuttgart.

Kwak, J. I., Nam, S.-H., Kim, S. W., Bajagain, R., Jeong, S.-W., An, Y.-J., 2019. Changes in soil properties after remediation influence the performance and survival of soil algae and earthworm. Ecotoxicology and Environmental Safety 174: 189-196.

Langhans, T., Storm, C., Schwabe, A., 2009. Community assembly of biological soil crusts of different successional stages in a temperate sand ecosystem, as assessed by direct determination and enrichment techniques. Microbial Ecology 58: 394–407.

Lukešová, A., 2001. Soil algae in brown coal and lignite post-mining areas in Central Europe (Czech Republic and Germany). Restoration Ecology 9: 341–350.

Lukešová, A., Komárek, J., 1987. Succession of soil algae on dumps from strip coal-mining in the Most Region (Czechoslovakia). Folia Geobotanica et Phytotaxonomica 22: 355–362.

Muñoz-Rojas, M., Román, R., Roncero Ramos, B., Erickson, T., Merritt, D., Aguila-Carricondo, P., Cantón, Y., 2018. Cyanobacteria inoculation enhances carbon sequestration in soil substrates used in dryland restoration. Science of The Total Environment 636: 1149–1154.

Oksanen, J., Blanchet, F.G., Kindt, R., Legendre, P., Minchin, P.R., O’Hara, R.B., Simpson, G.L., Solymos, P., Stevens, M.H.H., Wagner, H., 2015. Vegan: Community Ecology Package. R package version 2.2-1.

Pedrinho, A., Mendes, L., Pereira, A., Araujo, A., Vaishnav, A., Karpouzas, D., Singh, B., 2024. Soil microbial diversity plays an important role in resisting and restoring degraded ecosystems. Plant and Soil 500: 325–349.

R Core Team. 2025. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing Vienna, Austria. Available at [Access date: 20.01.2026]: https://www.r-project.org/

Rahmonov, O., Cabala, J., Bednarek, R., Rożek, D., Florkiewicz, A., 2015. Role of soil algae on the initial stages of soil formation in sandy polluted areas. Ecological Chemistry and Engineering S 22: 675–690.

Robinson, J.M., Liddicoat, C., Muñoz-Rojas, M., Breed, M.F., 2024. Restoring soil biodiversity. Current Biology 34: R393–R398

Schulz, K., Mikhailyuk, T., Dreßler, M., Leinweber, P., Karsten, Ulf., 2016. Biological Soil Crusts from Coastal Dunes at the Baltic Sea: Cyanobacterial and Algal Biodiversity and Related Soil Properties. Microbial Ecology 71: 178-193.

Stephan, K., Hubbart, J., 2022. Plant community, soil and microclimate attributes after 70 years of natural recovery of an abandoned limestone quarry. Land 12(1): 117.

Tarchevsky, V.V., Shtina, E.A., 1967. Development of algae on industrial dumps. In: Proceedings of the Interuniversity Conference. Kirov, pp. 146–150.

Wang, Z., Wang, G., Ren, T., Wang, H., Xu, Q., Zhang, G., 2021. Assessment of soil fertility degradation affected by mining disturbance and land use in a coalfield via machine learning. Ecological Indicators 125: 107608.

Wong, S.Y., Machado-de-Lima, N., Wilkins, D., Zhang, E., Ferrari, B.C., 2025. Fine-scale landscape heterogeneity drives microbial community structure at Robinson Ridge, East Antarctica. Science of the Total Environment 958: 177964.

Zhang X., Koehler, H. 2022. Soil algae for combating soil degradation – greenhouse experiment with different soil amendments. Soil Research 61: 70–82.

Zhang, B., Zhang, Y., Downing, A., Niu, Y., 2011. Distribution and composition of Cyanobacteria and microalgae associated with biological soil crusts in the Gurbantunggut desert, China. Arid Land Research and Management 25: 275-293.