Eurasian Journal of Soil Science

Volume 13, Issue 3, Jun 2024, Pages 202-209
DOI: 10.18393/ejss.1439846
Stable URL: http://ejss.fess.org/10.18393/ejss.1439846
Copyright © 2024 The authors and Federation of Eurasian Soil Science Societies



Assessing the impact of biofertilizer on soil microbial dynamics and metabolic activity in a controlled maize pot-grown experiment

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Dimitrova,K., Kaiyrbekov,T., Balabanova,D., 2024. Assessing the impact of biofertilizer on soil microbial dynamics and metabolic activity in a controlled maize pot-grown experiment. Eurasian J Soil Sci 13(3):202-209. DOI : 10.18393/ejss.1439846
Dimitrova,K.,Kaiyrbekov,T.,& Balabanova,D. Assessing the impact of biofertilizer on soil microbial dynamics and metabolic activity in a controlled maize pot-grown experiment Eurasian Journal of Soil Science, 13(3):202-209. DOI : 10.18393/ejss.1439846
Dimitrova,K.,Kaiyrbekov,T., and ,Balabanova,D."Assessing the impact of biofertilizer on soil microbial dynamics and metabolic activity in a controlled maize pot-grown experiment" Eurasian Journal of Soil Science, 13.3 (2024):202-209. DOI : 10.18393/ejss.1439846
Dimitrova,K.,Kaiyrbekov,T., and ,Balabanova,D. "Assessing the impact of biofertilizer on soil microbial dynamics and metabolic activity in a controlled maize pot-grown experiment" Eurasian Journal of Soil Science,13(Jun 2024):202-209 DOI : 10.18393/ejss.1439846
K,Dimitrova.T,Kaiyrbekov.D,Balabanova "Assessing the impact of biofertilizer on soil microbial dynamics and metabolic activity in a controlled maize pot-grown experiment" Eurasian J. Soil Sci, vol.13, no.3, pp.202-209 (Jun 2024), DOI : 10.18393/ejss.1439846
Dimitrova,Katya ;Kaiyrbekov,Tursynbek ;Balabanova,Dobrinka Assessing the impact of biofertilizer on soil microbial dynamics and metabolic activity in a controlled maize pot-grown experiment. Eurasian Journal of Soil Science, (2024),13.3:202-209. DOI : 10.18393/ejss.1439846

How to cite

Dimitrova, K., Kaiyrbekov, T., Balabanova, D., 2024. Assessing the impact of biofertilizer on soil microbial dynamics and metabolic activity in a controlled maize pot-grown experiment. Eurasian J. Soil Sci. 13(3): 202-209. DOI : 10.18393/ejss.1439846

Author information

Katya Dimitrova , Agricultural University – Plovdiv, Faculty of Plant Protection and Agroecology, Department of Microbiology and Environmental Biotechnologies, Plovdiv, Bulgaria
Tursynbek Kaiyrbekov , Agricultural University – Plovdiv, Faculty of Plant Protection and Agroecology, Department of Microbiology and Environmental Biotechnologies, Plovdiv, Bulgaria
Dobrinka Balabanova , Agricultural University – Plovdiv, Faculty of Agronomy, Department of Plant Physiology, Biochemistry and Genetics, Plovdiv, Bulgaria

Publication information

Article first published online : 19 Feb 2024
Manuscript Accepted : 11 Feb 2024
Manuscript Received: 15 Dec 2023
DOI: 10.18393/ejss.1439846
Stable URL: http://ejss.fesss.org/10.18393/ejss.1439846

Abstract

Biofertilizers, consisting of carefully selected microorganisms across various species and genera, exhibit distinct features that enhance soil fertility and promote plant growth. Embracing the principles of eco-friendly agriculture, the use of biofertilizers emerges as a pivotal strategy for sustainable farming, contributing to environmental preservation and the overall health and biodiversity of the soil. In this study, a commercially available biofertilizer, containing a specialized strain of Priestia megatherium with nitrogen-fixing capabilities, was employed alongside chemical fertilizers at two different doses (30 and 40 mg per kg of soil). The primary objective was to evaluate the impact of biofertilizer on the metabolic activity and structure of microbial communities in a short-term experiment involving potted maize plants, utilizing the BIOLOG® EcoPlates technique. Parameters such as average well-color development (AWCD) and substrate utilization across six guilds (SAWCD) were assessed to gauge microbial metabolic activity. Additionally, functional indexes, including Shannon diversity, Shannon evenness, and Simpson diversity, were calculated as indicators of soil microbial community functionality. While statistically significant differences in AWCD among the studied variants were not observed, all estimated functional indexes consistently revealed heightened microbial diversity and evenness following the application of biofertilizer. This noteworthy finding, achieved within a relatively short period of plant cultivation, underscores the necessity for further research to explore the biofertilizer's enduring effects on soil communities, both in controlled laboratory environments and under real-world field conditions.

Keywords

Biofertilizer, BIOLOG® EcoPlate, functional indexes, metabolic activity, microbial communities, Priestia megaterium.

Corresponding author

References

Adesemoye, A.O., Torbert, H.A., Kloepper, J.W., 2009. Plant growth-promoting rhizobacteria allow reduced application rates of chemical fertilizers. Microbial Ecology 58(4): 921-929.

Alef, K., 1995. Soil respiration. In: Methods in applied soil microbiology and biochemistry. Alef, K., Nannipieri, P. (Eds.). Harcourt Brace & Company, Academic Press, UK, pp. 215-216.

Al-Zubade, A., Phillips, T.D., Williams, M.A., Jacobsen, K.L., Van Sanford, D. 2021. Effect of biofertilizer in organic and conventional systems on growth, yield and baking quality of hard red winter wheat. Sustainability 13(24): 13861.

Baldi, E., Gioacchini, P., Montecchio, D., Mocali, S., Antonielli, L., Masoero, G., Toselli, M., 2021. Effect of biofertilizers application on soil biodiversity and litter degradation in a commercial apricot orchard. Agronomy 11(6): 1116.

Backer, R., Rokem, J.S., Ilangumaran, G., Lamont, J., Praslickova, D., Ricci, E., Subramanian, S., Smith, D.L., 2018. Plant growth-promoting rhizobacteria: Context, mechanisms of action, and roadmap to commercialization of biostimulants for sustainable agriculture. Frontiers in Plant Science 9: 1473.

Bargaz, A., Lyamlouli, K., Chtouki, M., Zeroual, Y., Dhiba, D., 2018. Soil microbial resources for ımproving fertilizers efficiency in an ıntegrated plant nutrient management system. Frontiers in Microbiology 9: 1606.

Cai, Y.F., Barber, P., Dell, B., O’Brien, P., Williams, N., Bowen, B., Hardy, G., 2010. Soil bacterial functional diversity is associated with the decline of Eucalyptus gomphocephala. Forest Ecology and Management 260(6): 1047–1057.

Centeno-Leija, S., Espinosa-Barrera, L., Velazquez-Cruz, B., Cárdenas-Conejo, Y., Virgen-Ortíz, R., Valencia-Cruz, G., Saenz, R.A., Marín-Tovar, Y., Gómez-Manzo, S., Hernández-Ochoa, B., Rocha-Ramirez, L.M., Zataraín-Palacios, R., Osuna-Castro, J.A., López-Munguía, A., Serrano-Posada, H., 2022. Mining for novel cyclomaltodextrin glucanotransferases unravels the carbohydrate metabolism pathway via cyclodextrins in Thermoanaerobacterales. Scientific Reports 12(1): 730.

Egner, H., Riehm, H., 1955. Die doppellaktatmethode (The double lactate method). In: Die Untersuchung von Böden. VDLUFA (Verbande Deutscher Landwirtschaftlicher Untersuchungs-und Forschungsanstalten), Methodenbuch Band 1. Hermann, T.R., Nuemann, K.E., (Eds.) Verlag, Radebeul and Berlin, Germany.

Ge, Z., Du, H., Gao, Y., Qiu, W., 2018. Analysis on metabolic functions of stored rice microbial communities by BIOLOG ECO microplates. Frontiers in Microbiology 9: 1375.

Hou, S., Ren, K., Fan, F., Zhao, M., Zhou, W., Zhou, B., Li, C., 2023. The effects of plant density and nitrogen fertilization on maize yield and soil microbial communities in the black soil region of Northeast China. Geoderma 430: 116325.

Huang, H.Y., Zhou, P., Shi, W.W., Liu, Q.L., Wang, N., Feng, H.W., Zhi, Y.E., 2012. Microbial functional diversity in facilities cultivation soils of nitrate accumulation. Procedia Environmental Sciences 13: 1037–1044.

Kurniawati, A., Stankovics, P., Hilmi, Y.S., Toth, G., Smol, M., Toth, Z., 2023. Understanding the future of bio-based fertilisers: The EU's policy and implementation. Sustainable Chemistry for Climate Action 3: 100033.

Lima, A.B., Cannavan, F.D.S., Germano, M.G., Dini-Andreote, F., Frabchini, J.C., de Paula, A.M., Teixeira, W.G.,Tsai, S.M., 2015. Effects of vegetation and seasonality on bacterial communities in Amazonian dark earth and adjacent soils. African Journal of Microbiology Research 9(40): 2119-2134.

Mehnaz, S., 2016. An overview of globally available bioformulations. In: Bioformulations: For sustainable agriculture. Arora, N.K, Mehnaz, S., Balestrini, R. (Eds.). Springer, pp. 267–281.

Ramakrishna, W., Yadav, R., Li, K., 2019. Plant growth promoting bacteria in agriculture: Two sides of a coin. Applied Soil Ecology 138: 10-18.

Roesti, D., Gaur, R., Johri, B., Imfeld, G., Sharma, S., Kawaljeet, K., Aragno, M., 2006. Plant growth stage, fertiliser management and bio-inoculation of arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria affect the rhizobacterial community structure in rain-fed wheat fields. Soil Biology and Biochemistry 38(5): 1111–1120.

Siswanti, D.U., Riesty, O.S., 2021. Effects of biofertilizer and manure application on growth rate and chlorophyll content of spinach (Amaranthus tricolor L.) under salinity stress condition. BIO Web of Conferences. The 1st International Conference of Advanced Veterinary Science and Technologies for Sustainable Development (ICAVESS 2021) 33: 05003.

Sofo, A., Ricciuti P.A., 2019. Standardized method for estimating the functional diversity of soil bacterial community by Biolog® EcoPlates™ assay - the case study of a sustainable olive orchard. Applied Sciences 9(19): 4035.

Stefanowicz, A., 2006. The Biolog plates technique as a tool in ecological studies of microbial communities. Polish Journal of Environmental Studies 15(5): 669-676.

Vessey, J.K., 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil 255: 571–586.

Wang, J., Liu, L., Gao, X., Hao, J., Wang, M., 2021. Elucidating the effect of biofertilizers on bacterial diversity in maize rhizosphere soil. PLoS One 16(4): e0249834.

Xiao, X., Zhu, Y., Gao, C., Zhang, Y., Gao, Y., Zhao, Y., 2022. Microbial inoculations improved rice yields by altering the presence of soil rare bacteria. Microbiological Research 254: 126910.

Abstract

Biofertilizers, consisting of carefully selected microorganisms across various species and genera, exhibit distinct features that enhance soil fertility and promote plant growth. Embracing the principles of eco-friendly agriculture, the use of biofertilizers emerges as a pivotal strategy for sustainable farming, contributing to environmental preservation and the overall health and biodiversity of the soil. In this study, a commercially available biofertilizer, containing a specialized strain of Priestia megatherium with nitrogen-fixing capabilities, was employed alongside chemical fertilizers at two different doses (30 and 40 mg per kg of soil). The primary objective was to evaluate the impact of biofertilizer on the metabolic activity and structure of microbial communities in a short-term experiment involving potted maize plants, utilizing the BIOLOG® EcoPlates technique. Parameters such as average well-color development (AWCD) and substrate utilization across six guilds (SAWCD) were assessed to gauge microbial metabolic activity. Additionally, functional indexes, including Shannon diversity, Shannon evenness, and Simpson diversity, were calculated as indicators of soil microbial community functionality. While statistically significant differences in AWCD among the studied variants were not observed, all estimated functional indexes consistently revealed heightened microbial diversity and evenness following the application of biofertilizer. This noteworthy finding, achieved within a relatively short period of plant cultivation, underscores the necessity for further research to explore the biofertilizer's enduring effects on soil communities, both in controlled laboratory environments and under real-world field conditions.

Keywords: Biofertilizer, BIOLOG® EcoPlate, functional indexes, metabolic activity, microbial communities, Priestia megaterium.

References

Adesemoye, A.O., Torbert, H.A., Kloepper, J.W., 2009. Plant growth-promoting rhizobacteria allow reduced application rates of chemical fertilizers. Microbial Ecology 58(4): 921-929.

Alef, K., 1995. Soil respiration. In: Methods in applied soil microbiology and biochemistry. Alef, K., Nannipieri, P. (Eds.). Harcourt Brace & Company, Academic Press, UK, pp. 215-216.

Al-Zubade, A., Phillips, T.D., Williams, M.A., Jacobsen, K.L., Van Sanford, D. 2021. Effect of biofertilizer in organic and conventional systems on growth, yield and baking quality of hard red winter wheat. Sustainability 13(24): 13861.

Baldi, E., Gioacchini, P., Montecchio, D., Mocali, S., Antonielli, L., Masoero, G., Toselli, M., 2021. Effect of biofertilizers application on soil biodiversity and litter degradation in a commercial apricot orchard. Agronomy 11(6): 1116.

Backer, R., Rokem, J.S., Ilangumaran, G., Lamont, J., Praslickova, D., Ricci, E., Subramanian, S., Smith, D.L., 2018. Plant growth-promoting rhizobacteria: Context, mechanisms of action, and roadmap to commercialization of biostimulants for sustainable agriculture. Frontiers in Plant Science 9: 1473.

Bargaz, A., Lyamlouli, K., Chtouki, M., Zeroual, Y., Dhiba, D., 2018. Soil microbial resources for ımproving fertilizers efficiency in an ıntegrated plant nutrient management system. Frontiers in Microbiology 9: 1606.

Cai, Y.F., Barber, P., Dell, B., O’Brien, P., Williams, N., Bowen, B., Hardy, G., 2010. Soil bacterial functional diversity is associated with the decline of Eucalyptus gomphocephala. Forest Ecology and Management 260(6): 1047–1057.

Centeno-Leija, S., Espinosa-Barrera, L., Velazquez-Cruz, B., Cárdenas-Conejo, Y., Virgen-Ortíz, R., Valencia-Cruz, G., Saenz, R.A., Marín-Tovar, Y., Gómez-Manzo, S., Hernández-Ochoa, B., Rocha-Ramirez, L.M., Zataraín-Palacios, R., Osuna-Castro, J.A., López-Munguía, A., Serrano-Posada, H., 2022. Mining for novel cyclomaltodextrin glucanotransferases unravels the carbohydrate metabolism pathway via cyclodextrins in Thermoanaerobacterales. Scientific Reports 12(1): 730.

Egner, H., Riehm, H., 1955. Die doppellaktatmethode (The double lactate method). In: Die Untersuchung von Böden. VDLUFA (Verbande Deutscher Landwirtschaftlicher Untersuchungs-und Forschungsanstalten), Methodenbuch Band 1. Hermann, T.R., Nuemann, K.E., (Eds.) Verlag, Radebeul and Berlin, Germany.

Ge, Z., Du, H., Gao, Y., Qiu, W., 2018. Analysis on metabolic functions of stored rice microbial communities by BIOLOG ECO microplates. Frontiers in Microbiology 9: 1375.

Hou, S., Ren, K., Fan, F., Zhao, M., Zhou, W., Zhou, B., Li, C., 2023. The effects of plant density and nitrogen fertilization on maize yield and soil microbial communities in the black soil region of Northeast China. Geoderma 430: 116325.

Huang, H.Y., Zhou, P., Shi, W.W., Liu, Q.L., Wang, N., Feng, H.W., Zhi, Y.E., 2012. Microbial functional diversity in facilities cultivation soils of nitrate accumulation. Procedia Environmental Sciences 13: 1037–1044.

Kurniawati, A., Stankovics, P., Hilmi, Y.S., Toth, G., Smol, M., Toth, Z., 2023. Understanding the future of bio-based fertilisers: The EU's policy and implementation. Sustainable Chemistry for Climate Action 3: 100033.

Lima, A.B., Cannavan, F.D.S., Germano, M.G., Dini-Andreote, F., Frabchini, J.C., de Paula, A.M., Teixeira, W.G.,Tsai, S.M., 2015. Effects of vegetation and seasonality on bacterial communities in Amazonian dark earth and adjacent soils. African Journal of Microbiology Research 9(40): 2119-2134.

Mehnaz, S., 2016. An overview of globally available bioformulations. In: Bioformulations: For sustainable agriculture. Arora, N.K, Mehnaz, S., Balestrini, R. (Eds.). Springer, pp. 267–281.

Ramakrishna, W., Yadav, R., Li, K., 2019. Plant growth promoting bacteria in agriculture: Two sides of a coin. Applied Soil Ecology 138: 10-18.

Roesti, D., Gaur, R., Johri, B., Imfeld, G., Sharma, S., Kawaljeet, K., Aragno, M., 2006. Plant growth stage, fertiliser management and bio-inoculation of arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria affect the rhizobacterial community structure in rain-fed wheat fields. Soil Biology and Biochemistry 38(5): 1111–1120.

Siswanti, D.U., Riesty, O.S., 2021. Effects of biofertilizer and manure application on growth rate and chlorophyll content of spinach (Amaranthus tricolor L.) under salinity stress condition. BIO Web of Conferences. The 1st International Conference of Advanced Veterinary Science and Technologies for Sustainable Development (ICAVESS 2021) 33: 05003.

Sofo, A., Ricciuti P.A., 2019. Standardized method for estimating the functional diversity of soil bacterial community by Biolog® EcoPlates™ assay - the case study of a sustainable olive orchard. Applied Sciences 9(19): 4035.

Stefanowicz, A., 2006. The Biolog plates technique as a tool in ecological studies of microbial communities. Polish Journal of Environmental Studies 15(5): 669-676.

Vessey, J.K., 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil 255: 571–586.

Wang, J., Liu, L., Gao, X., Hao, J., Wang, M., 2021. Elucidating the effect of biofertilizers on bacterial diversity in maize rhizosphere soil. PLoS One 16(4): e0249834.

Xiao, X., Zhu, Y., Gao, C., Zhang, Y., Gao, Y., Zhao, Y., 2022. Microbial inoculations improved rice yields by altering the presence of soil rare bacteria. Microbiological Research 254: 126910.



Eurasian Journal of Soil Science